Bovine collagen peptides, Type I and III
DiTriX uses its own collagenase enzyme to cleave collagen inside its Gly-X-Y repeat. The result is a range from everyday collagen peptides to tripeptide grades with up to 75% collagen tripeptide content.
Both product groups start from traceable bovine hide and are hydrolysed on our own enzyme platform. They differ in process, peptide size and the job they do in your product.
Collagen is a long chain of Gly-X-Y repeats. Conventional hydrolysis breaks it at random into peptides of 20 to 100 amino acids. Our collagenase cuts inside the repeat, so a large share of the product ends up as intact di- and tripeptides.
Collagenase releases short sequences such as Gly-Pro-Hyp, Pro-Hyp, Gly-Pro-Ala and Gly-Pro-Pro.
Di- and tripeptides resist further digestion and are carried across the gut wall by the PEPT1 transporter.
Human studies detect Gly-Pro-Hyp and Pro-Hyp in blood within 30 to 60 minutes of intake.
These peptides are studied for their role in fibroblast activity and collagen synthesis in skin and connective tissue.
Molecular weight distribution of the three Collagen Tripeptide grades, measured by SEC-HPLC. The higher the grade, the more of the product sits below 1,000 Da.
Each area is supported by published research on collagen peptides and collagen tripeptides. Claim wording has to follow the rules of your market; our team can help you match claims to evidence.
Human trials with 1 g a day of collagen tripeptide report better skin hydration and lower transepidermal water loss after 12 weeks.
Studies of low molecular weight collagen peptides report less joint discomfort and better function in adults with knee complaints.
Daily collagen helps formulate products for adults who want to keep skin, bone and connective tissue in good shape as they age.
Collagen peptides combined with training are studied for tendon remodelling and functional recovery in active adults.
| Format | Recommended | Why |
|---|---|---|
| Clear and functional waters | Collagen Tripeptide | ≥95% transmittance for transparent bottles |
| Beauty shots and sachets | Collagen Tripeptide 50+ or 75+ | Gram-level doses with a high tripeptide share |
| Joint-health supplements | Collagen Tripeptide STD or 50+ | Tripeptide content at a price that suits daily dosing |
| Protein powders and shakes | Collagen Peptide | High protein density for nutrition claims |
| Bars, bakery and cereals | Collagen Peptide | Stable through baking and extrusion |
| Capsules and tablets | Collagen Tripeptide 75+ | Most tripeptide per milligram where space is tight |
DiTriX is produced on our own collagenase enzyme platform. Because we design and produce the enzyme ourselves, we control where the protein chain is cut, batch after batch. That is what keeps the peptide profile on the specification sheet consistent with what arrives in your plant.
Every DiTriX product ships in its own colour-coded 15 kg kraft bag with a PE inner liner. Stored cool, dry and sealed, shelf life is 5 years.
Tell us what you are making. Our application team replies with the grade and dose we would start with, a specification sheet and a sample.
info@ditrix.comDi- and tripeptide-rich bovine collagen for premium products where absorption is the story. Available in three grades, from 25% to 75% collagen tripeptide content.
≥25%
collagen tripeptide content
Avg. MW ~2,000 Da. The entry grade for daily collagen products that want a tripeptide claim.
≥50%
collagen tripeptide content
Avg. MW ~1,500 Da. A balance of tripeptide content and cost for beauty and joint products.
≥75%
collagen tripeptide content
Avg. MW below 1,000 Da. The most tripeptide per gram, for shots, sachets and capsules.
Share of peptides in each molecular weight band, measured by SEC-HPLC. Grade 75+ has half of its mass below 500 Da, the range where di- and tripeptides sit.
| Source | Bovine hide, Type I and III |
|---|---|
| Process | Targeted collagenase hydrolysis |
| Avg. molecular weight | <1,000 Da |
| Collagen tripeptide (CTP) | ≥75% |
| Gly-Pro-Hyp | ~10% |
| Protein, dry basis | ≥90% |
| Transmittance in solution | ≥95% |
| Packaging | 15 kg kraft bag, PE inner liner |
| Shelf life | 5 years, cool, dry, sealed |
Moisture, ash, pH and microbiology limits are on the full specification sheet.
| Glycine | 16.759 | Asparagine | 1.657 |
|---|---|---|---|
| Proline | 11.590 | Threonine | 1.592 |
| Glutamic acid | 11.579 | Isoleucine | 1.430 |
| Hydroxyproline | 9.784 | Histidine | 0.547 |
| Alanine | 7.558 | Tyrosine | 0.514 |
| Arginine | 7.009 | Methionine | 0.376 |
| Lysine | 3.195 | Tryptophan | <0.046 |
| Aspartic acid | 3.121 | Citrulline | <0.023 |
| Leucine | 3.022 | Cystine | <0.020 |
| Serine | 2.360 | Sarcosine | <0.015 |
| Valine | 1.861 | Ornithine | <0.013 |
| Phenylalanine | 1.753 |
Profiles for the other grades and product groups on request.
Send us your format and target dose. We will suggest a grade and send a sample with its specification.
A summary of published human and preclinical studies on collagen tripeptides and low molecular weight collagen peptides. These studies used ingredients from other producers; they describe the ingredient class, not DiTriX finished products.
Absorption and skin are backed by human trials. Bone and wound healing rest mostly on animal and lab work. Metabolic effects are early-stage and are not part of our positioning.
| Area | Type of evidence | What was found | Status |
|---|---|---|---|
| Absorption | Human pharmacokinetics | Gly-Pro-Hyp and Pro-Hyp reach plasma intact, peaking 30 to 60 minutes after intake | Established |
| Skin hydration and barrier | Randomised controlled trials | Higher skin hydration and lower water loss with 1 g a day over 12 weeks | Established |
| Skin elasticity and wrinkles | Randomised controlled trials | Improved elasticity and wrinkle measures with low molecular weight collagen peptide | Established |
| Joint comfort | Randomised controlled trials, mostly collagen peptides | Less knee discomfort and better function over 3 to 6 months | Supported |
| Bone and connective tissue | Animal studies | More osteoblasts and faster defect filling in rats given collagen tripeptide orally | Preclinical |
| Metabolic effects | Animal and pilot studies | Early signals on glucose and appetite regulation | Exploratory |
Yamamoto et al., 2016, Biological and Pharmaceutical Bulletin
After a single dose of collagen tripeptide, Gly-Pro-Hyp and Pro-Hyp were measured intact in plasma. Peak Gly-Pro-Hyp was far higher than after a conventional collagen peptide.
doi.org/10.1248/bpb.b15-00624Tak et al., 2021, Frontiers in Medicine
A randomised, double-blind, placebo-controlled trial in middle-aged women found better skin hydration with collagen tripeptide. It was well tolerated.
doi.org/10.3389/fmed.2020.608903Kim et al., 2018, Nutrients
A randomised, double-blind, placebo-controlled study of low molecular weight collagen peptide at 1 g a day reported improvements in all three skin measures.
doi.org/10.3390/nu10070826Park et al., 2025, Frontiers in Nutrition
A randomised, placebo-controlled trial of low molecular weight collagen peptides at 3 g a day in knee osteoarthritis assessed efficacy and safety; no serious adverse events were reported.
doi.org/10.3389/fnut.2025.1644899Ichikawa et al., 2010, Int. J. Food Sciences and Nutrition
Hydroxyproline-containing di- and tripeptides were found at high concentration in blood after oral intake of collagen hydrolysate.
doi.org/10.3109/09637480903257711Bischof et al., 2024, Sports Medicine
A systematic review and meta-analysis of collagen peptide supplementation combined with long-term training, covering strength, tendon remodelling and recovery.
doi.org/10.1007/s40279-024-02079-0Across human trials at 1 to 10 g a day for up to 24 weeks, no serious adverse events were linked to collagen peptides or tripeptides. Reported effects were mostly mild and short-lived digestive complaints, and laboratory safety values stayed within normal ranges.
We recommend standard label advice for pregnancy and advanced kidney disease, as for any protein ingredient.
Example structure and function wording, each paired with the evidence behind it. Final wording depends on your market.
In the European Union no health claim for collagen is authorised under Regulation (EC) No 1924/2006. Avoid wording that implies treating or preventing disease in every market.
Our clinical summary covers 30+ references, study designs, doses and adverse event tables.
